Chapter 9: Biotechnology : Principles and Processes MCQs | biology Class 12 CBSE

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Chapter 9: Biotechnology : Principles and Processes MCQs | biology Class 12 CBSE | ByteNotes.in

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Question 1

Objective

Which of the following scientists isolated the antibiotic resistance gene and ligated it into a plasmid vector to create the first recombinant DNA molecule?

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Medium

Question 2

Objective

The first restriction endonuclease to be isolated and characterised was:

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Medium

Question 3

Objective

In the name 'EcoRI', what does the letter 'R' represent?

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Easy

Question 4

Objective

Restriction endonucleases cut DNA at specific positions known as:

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Easy

Question 5

Objective

The single-stranded overhanging ends produced after restriction enzyme digestion are called:

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Easy

Question 6

Objective

Which enzyme is used to join two DNA fragments to produce a recombinant DNA molecule?

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Medium

Question 7

Objective

Agarose used as the matrix in gel electrophoresis is extracted from:

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Question 8

Objective

In gel electrophoresis, DNA fragments are visualised by staining with:

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Medium

Question 9

Objective

In gel electrophoresis, which end of the gel would you load the DNA sample?

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Medium

Question 10

Objective

The process by which a desired DNA band is extracted from an agarose gel is called:

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Medium

Question 11

Objective

Which feature of a cloning vector is responsible for controlling the number of copies of the vector in the host cell?

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Hard

Question 12

Objective

In pBR322, the foreign DNA is inserted at the BamHI site within the tetracycline resistance gene. A recombinant bacterium will show which pattern of antibiotic resistance?

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Easy

Question 13

Objective

In blue-white selection, bacteria containing a recombinant plasmid (with an insert in the lacZ gene) form which colour of colonies on X-gal medium?

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Easy

Question 14

Objective

The Ti plasmid used as a cloning vector in plants belongs to which organism?

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Easy

Question 15

Objective

Which chemical treatment is used to make bacterial cells competent for DNA uptake?

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Medium

Question 16

Objective

In micro-injection, foreign DNA is directly introduced into the:

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Medium

Question 17

Objective

The biolistics method (gene gun) uses microparticles coated with DNA made of which metals?

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Easy

Question 18

Objective

PCR stands for:

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Medium

Question 19

Objective

The thermostable DNA polymerase used in PCR is isolated from:

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Easy

Question 20

Objective

In a PCR reaction, which step involves the breaking apart of the double-stranded DNA template?

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Chapter 9: Biotechnology : Principles and Processes | MCQs

Which of the following scientists isolated the antibiotic resistance gene and ligated it into a plasmid vector to create the first recombinant DNA molecule?

The first restriction endonuclease to be isolated and characterised was:

In the name 'EcoRI', what does the letter 'R' represent?

Restriction endonucleases cut DNA at specific positions known as:

The single-stranded overhanging ends produced after restriction enzyme digestion are called:

Which enzyme is used to join two DNA fragments to produce a recombinant DNA molecule?

Agarose used as the matrix in gel electrophoresis is extracted from:

In gel electrophoresis, DNA fragments are visualised by staining with:

In gel electrophoresis, which end of the gel would you load the DNA sample?

The process by which a desired DNA band is extracted from an agarose gel is called:

Which feature of a cloning vector is responsible for controlling the number of copies of the vector in the host cell?

In pBR322, the foreign DNA is inserted at the BamHI site within the tetracycline resistance gene. A recombinant bacterium will show which pattern of antibiotic resistance?

In blue-white selection, bacteria containing a recombinant plasmid (with an insert in the lacZ gene) form which colour of colonies on X-gal medium?

The Ti plasmid used as a cloning vector in plants belongs to which organism?

Which chemical treatment is used to make bacterial cells competent for DNA uptake?

In micro-injection, foreign DNA is directly introduced into the:

The biolistics method (gene gun) uses microparticles coated with DNA made of which metals?

PCR stands for:

The thermostable DNA polymerase used in PCR is isolated from:

In a PCR reaction, which step involves the breaking apart of the double-stranded DNA template?

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